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5.2 The DMT Microscope

The DMT microscope is an invert microscope equipped with a Zeiss Achromat 10X

/ 0.25 objective and build-in CCD camera. The objective is adjustable in all X Y Z

directions using the three micro positioners on the front as illustrated in Fig. 5.5.

The VediView

™

data acquisition and analysis software obtains an image by measuring

the differences in light intensity passing through the walls of a vessel segment

mounted in the culture chamber. Traditionally a white light source is sufcient for

such a purpose, but has one major disadvantage. A white light source makes the

data acquisition and analysis very sensitive to changes in white light intensity from

the surroundings, such as ambient light and sunlight. To avoid the surroundings

inuencing the data acquisition and analysis, the DMT microscope has a built-in

infrared light source.

Figure 5.5 How to adjust the position and focus of the microscope in the X Y Z directions.

User Manual vers. 1.4 Chapter 5

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DMT Presenter Control Manual do Utilizador Página 45