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Culture Myograph System Model 202CM

2.3.3 Setup and Control of Superfusion Flow

The superfusion circuit consists of an inow and an outow from the culture

myograph chamber. Both ows are driven by the same peristaltic pump, which leads

the superfusion buffer from the reservoir to the culture myograph chamber and nally

to a waste bottle. The small steel pipe on the culture myograph chamber cover is

connected to a sterile micro lter (0.20µm). The small pipe works as a breathing valve

to prevent over pressure in the culture myograph chamber.

The superfusion buffer reservoir is continuously aerated with a mixture of 95%

and 5% CO

. The gas passes through a sterile micro lter (0.20µm) to prevent

contamination of the sterile superfusion buffer.

Peristaltic Pump

Waste Bottle

Culture Myograph Chamber

Oxygen

Supply

Superfusion

Buffer

Micro Filters

Figure 2.4 Illustration of perfusion ow control

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