DMT Presenter Control Manual do Utilizador

User ManualVersion 1.5Culture Myograph System Model 202CM

10Culture Myograph System Model 202CM Unpacking the Myograph SystemPlease take a few minutes to carefully inspect your new Culture Myograph Syst

11User Manual vers. 1.4 Chapter 1Cable to myo-InterfaceVertical regulation plate for left glass cannulaFixation and longitu-dinal regulation pla

Plug for connec-tion of tempera-ture probeLight intensity regulation plugInfrared light scource Zeiss Achromat 10X / 0.25 objectiveLever to fasten cul

Chapter 2 - Setting up2.1 The Complete Culture Myograph System – 202CMFigure 2.1 Setting up step by step Note:If you have purchased a computer from DM

14Culture Myograph System Model 202CM 2.2.1 Setting up the Complete Culture Myograph System 202CMThis section describes how to connect the cable

2.2.2.1 Software and Picolo Driver Installation Insert the Culture Myograph 202CM installation CD, to install the 202CM Control program for the Heat

Click “Install”The 202CM Control program is now installing from the CD.Click “Finish”The installation will now proceed with installation of the Picolo

Ignore the warning.Click “OK”It is recommended to install the software in the default folder.Click “Next”.Setup Type, choose Typical.Click “Next”Click

18Culture Myograph System Model 202CM Click “Yes”The Picolo Frame Grabber is now installing from the CD.The Picolo Frame Grabber is now installe

It is recommended to install the software in the default folder.Click “Next”Click “Next”Click “Next”The VediView Software package is now installed.Cli

2Culture Myograph 202CM

20Culture Myograph System Model 202CM 2.2.2.2 DMT 202CM VCP driver installationConnect the Heat Controller to the computer’s USB port, using a s

Note:A sterile micro lter (0.20µm) is connected between the pressure manometer reservoir and the two perfusion reservoirs to prevent contamination of

22Culture Myograph System Model 202CM 2.3.3 Setup and Control of Superfusion Flow The superfusion circuit consists of an inow and an outow fro

Chapter 3 - VediView™ Software Manual3.1 Introduction to VediView™VediView™ provides an interface for image analysis of small blood vessels mounted in

24Culture Myograph System Model 202CM 3.2 User Interface3.2.1 MenusMenus are located across the top of the VediView™ interface. All the actions

3.2.3 ToolbarVediView™ contains a "dockable" tool bar containing buttons with specic icons used as short cuts to various VediView™operation

3.2.4 Main WindowThe main window can show any of the graphical displays of the control bars, which are the frame grabber image, data control or analys

3.2.5 ModalityMany aspects of the VediView™ software layout are similar to standard Windows applications (e.g. Microsoft Ofce). Main menus are availa

28Culture Myograph System Model 202CM All control bars can be “undocked” and re-sized for convenience in a given experiment. Once the control ba

3.3.2.1 Displaying ImagesThe real time frame-grabbing mode may be switched on or off by means of the “start/stop display” button or from the pop-up

Culture Myograph System - Model 202CMUser ManualTrademarksPentium is a registered trademark of the Intel Corporation. Windows, Windows 95, Windows 98,

30Culture Myograph System Model 202CM 3.3.2.6 Distance MeasurementIf the user wishes to measure a distance on the image, VediView™ can supply th

3.3.3.1 Selecting Data for DisplayThe data control bar can be used to display up to six parameters in the interface. These can be selected from the li

32Culture Myograph System Model 202CM Data logging is not automatic and must be activated by selecting (or creating) a le. Files are chosen by

3.3.4.2 Calibration of Image AnalysisCalibration of image dimensional analysis can be done in sensible units (e.g. microns) dened by the user. To per

34Culture Myograph System Model 202CM If the analysis rectangle contains a “valid” vessel but only has the outer edges determined by edge detect

VediView can analyse vessels positioned in either the vertical or horizontal planes. The congure analysis dialog box can be used to select the appro

36Culture Myograph System Model 202CM 5. Once the dimensions and units of the calibrated rectangle are correctly entered, click the ”calibr

interface is done through reconguration of the control bars and main window contents. In the example shown in Fig. 3.28 the data control bar has be

38Culture Myograph System Model 202CM 3.6 FAQThere are only six parameters in the graphical display, how do I display other data acquired or cal

Chapter 4 - DMT 202CM Control Program4.1 Control of Temperature and Light IntensityIn addition to the VediView™data acquisition and analysis software

4Culture Myograph System Model 202CM SafetyThe Culture Myograph System has been designed for use only in teaching and research applications. It

Set-temperature:The temperature controller tab is used to adjust the set temperature for each chamber (range: 10 - 450C with 0.10C accuracy). If a tem

Chapter 5 - Culture Myograph Manual5.1 The Culture Myograph UnitThe culture myograph unit is placed on the DMT microscope as illustrated in Fig. 5.1.

Horizontal Adjustment:Horizontal adjustment is performed as illustrated in Fig. 5.1 and 5.2.Gently loosen the screw xing the chamber and carefully mo

5.1.2 Removal and Mounting of Glass Cannulas and ChamberReplacement of glass cannulas and demounting of the culture myograph chamber for sterilisation

44Culture Myograph System Model 202CM 5.1.3 Changing O-ringsEach locknut on the culture myograph chamber is equipped with rubber O-rings (ø1.07

5.2 The DMT MicroscopeThe DMT microscope is an invert microscope equipped with a Zeiss Achromat 10X / 0.25 objective and build-in CCD camera. The obje

5.3 Pressure Regulator The Pressure Regulator is connected to the culture myograph system to generate a pressure on the mounted vessel from the perfus

In cases of red or brown discolorations appearing on the chamber sides, the following cleaning procedure will work in most cases:1. Incubate the myog

48Culture Myograph System Model 202CM Chapter 6 - Myograph Accessories and Spare PartsThis chapter contains a complete list of equipment needed

6.2 Culture Myograph 202CM System AccessoriesThis section contains a list of special accessories available for the Culture Myograph System - Model 202

EMC / EMIThis equipment has been tested and found to comply with the limits for a Class B Digital device, pursuant to part 15 of the FCC rules. These

50Culture Myograph System Model 202CM Chapter 7 - Getting Started7.1 Dissection Protocol for Small Mesenteric ArteriesThe culture myograph techn

51User Manual vers. 1.4 Chapter 76. Use high quality forceps and ocular dissection scissors (see Chapter 6.1) to dissect the vessel segmen

52Culture Myograph System Model 202CM • The branch points of arteries are V-shaped whereas those of veins are more U-shaped.• The arterial w

53User Manual vers. 1.4 Chapter 79. Clean the artery by removing any remaining adipose or connective tissue. Gently pull away adipose or c

54Culture Myograph System Model 202CM 7.2 Mounting Protocol for Small ArteriesThis section shortly describes the basic technique of mounting sm

55User Manual vers. 1.4 Chapter 77.3 Buffer Recipes7.3.1 Physiological Saline Solution (PSS)1x PSS (1000ml):Solution 1.Chemical MW (g/mol)

56Culture Myograph System Model 202CM 25x Concentrated PSS (1000ml)Solution 1Chemical MW (g/mol) Conc. (mmol/l) Conc. (g/l)NaCl 58

Appendix 1 - Terms of WarrantyWarrantyDMT A/S warrants to the original user that myograph systems manufactured by DMT A/S will be free from defects in

58Culture Myograph System Model 202CM Appendix 2 - Service CheckA myograph working at optimal performance is extremely important for success whe

Appendix 3 - Shipping InstructionsIf the myograph system needs to be sending back for service or repair, please read the following shipping instructio

6Culture Myograph System Model 202CM Certicate of ConformityDMT A/S, Skejbyparken 152, 8200 Aarhus N., Denmark, hereby declares its responsibil

Appendix 4 - System Specications Chamber Unit:Vessel size: Chamber:Chamber volume: Capillary ports:Superfusion ports:Base window: Chamber material:Ch

Appendix 5 - Fuse ChangingThe main fuse of the myograph system is placed inside the power inlet on the Heat controller. If the fuse blows it is easily

Appendix 6 - VCP – Driver InstallationThe rst time the 202CM Heat Controller and the DMT microscope is connected to the PC the VCP drivers must be in

If Windows XP is congured to warn when unsigned (non-WHQL certied) drivers are about to be installed, the following screen will be displayed.Click

When the drivers for the Heat controller and the microscope are installed the 202CM Controller Software can be opened and connected as described in pa

DMT-USA, Inc.P.O. Box 681373Marietta, GA 30068-0023USA Tel.: +1 770 612 8014Fax: +1 678 302 [email protected] or sales@dmt-us

7User Manual vers. 1.4 ContentsContentsSafety ...

8Culture Myograph System Model 202CM 3.6 FAQ .......

About this ManualThis manual contains a complete list of procedures describing how to install, maintain and get started using the Culture Myograph Sys